마우스 배아 1차 배양에서 배아 섬유아세포 분리

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Mouse embryonic fibroblasts

강점

엄격한 품질관리 및 품질보장: 세포가 깨끗하고 오염이 없도록 박테리아, 진균, 미코플라스마 검사를 수행함

풍부한 경험: 수십 년 동안 배아 섬유아세포를 마련해온 경험

다양한 제품 보유: 자체 사용 및 외부 고객을 위해 네오마이신, 하이그로마이신, 퓨로마이신으로 조작한 마우스 배아 섬유아세포를 보유

실험 과정 요약

재태 연령 E13.5일에 마우스 배아를 수집합니다. 줄기 조직을 킬레이트제(보통 EDTA) 존재 하에서 효소 분해하여 배아 섬유아세포를 단일세포로 얻습니다. 이것을 MEF 성장 배지에서 배양하면 섬유아세포가 생존, 성장, 증식할 수 있습니다.

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Isolation of fibroblasts from mouse embryos and preparation of feeder layer cells

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Mouse embryos at 13.5-day gestational age

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Mouse embryonic fibroblasts

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